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American Society of Microbiology ABMM Sample Questions:

1. A Gram stain of anaerobic growth from a peritoneal fluid abscess reveals pleomorphic, pale-staining Gram- negative rods. The isolate grows on Bacteroides Bile Esculin (BBE) agar, hydrolyzing esculin (producing black colonies), and is resistant to kanamycin (1000 µg), vancomycin (5 µg), and colistin (10 µg) disks. Which organism group is most likely?

A) Bacteroides fragilis group
B) Fusobacterium nucleatum
C) Clostridium perfringens
D) Prevotella melaninogenica group

2. A Gram stain of cerebrospinal fluid (CSF) from a 2-year-old child with meningitis shows small, pleomorphic Gram-negative coccobacilli. Culture on chocolate agar yields growth, but there is no growth on standard 5% sheep blood agar unless streaked near a colony of Staphylococcus aureus. This phenomenon indicates a requirement for which growth factor(s)?

A) Factor X (hemin) only
B) Both Factor X and Factor V
C) Factor V (NAD) only
D) Cysteine and pyridoxal

3. A clinical microbiology laboratory is preparing a Gram stain of a mixed bacterial culture containing both Gram-positive and Gram-negative organisms. The correct order of reagents used in the Gram staining procedure is:

A) Iodine, crystal violet, decolorizer, safranin
B) Decolorizer, crystal violet, iodine, safranin
C) Safranin, crystal violet, decolorizer, iodine
D) Crystal violet, iodine, decolorizer, safranin

4. A clinical microbiology laboratory encounters a Gram-negative bacillus isolated from a bloodstream infection. The isolate exhibits resistance to multiple antibiotics, including carbapenems. Further phenotypic testing reveals the production of a metallo-beta-lactamase (MBL). Which of the following antibiotic combinations might still retain activity against this isolate?

A) Piperacillin-tazobactam
B) Aztreonam
C) Aztreonam-avibactam
D) Ceftazidime-avibactam

5. A researcher is investigating the mechanism of action of a novel antibacterial agent that inhibits bacterial protein synthesis. The agent binds to the 50S ribosomal subunit and prevents the translocation of peptidyl- tRNA. This mechanism of action is similar to which of the following antibiotics?

A) Penicillin
B) Azithromycin
C) Ciprofloxacin
D) Vancomycin

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